Thedevelopmentofinnovativeskincareproductsrequiresadvanced,human-relevantinvitrotestingtechnologies.WhenworldleADIngpersonalcareandcosmeticscientistsevaluatenovelingredientsandformulations,theychoosetheEpiDermFT3Dhumanskinmodel.
- LargeDonorInventorywithMatchedKeratinocytesandFibroblasts
- AdultorNeonatalDonorsareAvailable
- DeliveredReady-to-Use
Technology:
Engineeredtoenableinvitroskinresearchinwhichfibroblast-keratinocytecellinteractionsareimportant,MatTek’sEpiDermFTsystemconsistsofnormal,humanepidermalkeratinocytes(NHEK)andnormal,humandermalfibroblasts(NHFB)culturedtoformamultilayeredmodelofthehumandermisandepidermis.Culturedattheair-liquidinterfaceineasy-to-handletissuecultureinserts,EpiDermFTattainslevelsofdifferentiationatthecuttingedgeoftissueengineeringtechnology.
EpiDermFTconsistsoforganizedkeratin5expressingbasalcells,involucrinandkeratin10expressingspinousandgranularlayers,andcornifiedepidermallayersanalogoustothosefoundinvivo.Thedermalcompartmentiscomposedofacollagenmatrixcontainingviablenormalhumandermalfibroblasts(NHDF).Theepidermalanddermallayersaremitoticallyandmetabolicallyactiveandexhibitinvivo-likemorphologicalandgrowthcharacteristicswhichareuniformandhighlyreproducIBLe.
Awell-developedbasementmembraneispresentatthedermal/epidermaljunction.Hemidesomosomes(›),laminalucida,laminadensa(→)andanchoringfibrilstructures(»)areevidentbytransmissionelectronmicroscopy.
Applications:
EpiDermFTtissuesareusedacrossawiderangeofapplicationsincludingscientificefficacyandclaimssubstantiation.Duetoadryskinsurface,EpiDermFTcanbeusedtoevaluateBIOLOGicalresponsestotopicalapplicationsofformulationsoringredients.Simpleprotocolsandtheevaluationofearlymolecularendpointsallowresearchtoacquiredataindays,notweeksormonths.
Anti-Aging
Examinechangesinextracellularmatrixgeneandproteinexpressionandhistologicalstructuretodeterminetheanti-agingefficacyofcosmeticingredientsorfinalformulation.EpiDermFTisavailablefromarangeofadultdonors.
Anti-AgingApplicationNote
WoundHealing
UseEpiDermFTtoevaluatetherapeuticswiththewoundhealingassaykit.Woundhealingassaykitsinclude24pre-woundedtissuesandareavailablewithepidermalonlywoundsorfullthicknesswoundsoftheepidermisanddermis.
RecentWoundHealingPoster
SkinHydration
UseEpiDermFTtoassesstheefficacyoftopicallyappliedmoisturizersbymeasuringtheelectricalimpedanceoftheepidermis.Amenabletocreams,gel,lotionsorotherformulations,theSkinHydrationassayallowsfordataanalysiswithin24hours.
SkinHydrationApplicationNote
UVProtection
Analyzecyclopyrimidinedimerformation,inflammatorymediatorreleaseandoxidativeendpointstoevaluatetheUVprotectiveefficacyofcompoundsorformulations.
UVProtection ApplicationNote
BrowseourreferencelibrarytoseehowourEpiDermFT tissuehasbeenusedintheseareasofstudy.
TechSpecs:
Tissue
Kit: AstandardEpiDermFTkit(EFT-400)consistsof24tissues.(Tissue“kits”containtissues,asmallamountofculturemedium,andplasticware;contactMatTekforspecifickitcontents)
Substrate: CostarSnapwell™singlewelltissuecultureplateinserts areused.Poresize=0.4μm,Diameter=1.2cm.Surfacearea=1.0cm2
Culture: Air-liquidinterface
Histology: 8-12celllayersplusstratumcorneum(basal,spinous,andgranularlayers)
Lotnumbers: Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualkitswithinagivenlotoftissues.Alltissuekitswithinalotareidenticalinregardstocells,medium,handling,cultureconditions,etc.
Shipment: At4°Conmedium-supplemented,agarosegelsin6-wellplates
Shipmentday: EveryMonday.ShipmentonThursdayalsopossibleuponspecialrequest
Delivery: TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-Thursdaydependingonlocation
Shelflife: Includingtimeintransit,tissuesmaybestoredat4°Cforupto6dayspriortouse.However,extendedstorageperiodsarenotrecommendedunlessnecessary.Inaddition,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning)
Lengthofexperiments: Culturescanbecontinuedforupto2weekswithgoodretentionofnormalepidermalmorphology.Culturesmustbefedeveryotherdaywith5.0mlofEFT-400medium
Cells
Type: Normalhumanepidermalkeratinocytes(NHEK);Normalhumandermalfibroblasts(NHDF)
Geneticmake-up: Singledonor
Derivedfrom: Neonatal-foreskintissue(NHEK);Neonatalskin(NHDF)
Alternatives: NHEKfromadultbreastskin;NHDFfromadultskin
Screenedfor: HIV,Hepatitis-B,Hepatitis-C,mycoplasma
Medium
Basemedium: Dulbecco’sModifiedEagle’sMedium(DMEM)
Growthfactors/hormones: Epidermalgrowthfactor,insulin,hydrocortisoneandotherproprietarystimulatorsofepidermaldifferentiation
Serum: None
Antibiotics: Gentamicin5µg/ml(10%ofnormalgentamicinlevel)
Anti-fungalagent: AmphotericinB0.25µg/ml
pHIndicator: Phenolred
Otheradditives: Lipidprecursorsusedtoenhanceepidermalbarrierformation(proprietary)
Alternatives: Phenolred-free(EFT-400-PRF),antibiotic-free(EFT-400-ABF),anti-fungal-free(EFT-400-AFF),orhydrocortisone-freemediumandtissue(EFT-400-HCF)areavailable.Agentsareremovedatleast3dayspriortoshipment
Assay/Maintenancemedium: EFT-400-ASYisutilizedforassays;EFT-400-MMisusedfortermmaintenanceoftheEFT-400tissues
QualityControlandSterility
Visualinspection: Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipment
End-usetesting: Tissuesareexposedto1%TritonX-100for8,12,16and24hours.ThetimeofexposurerequiredtoreducethetissueviABIlity(ET-50)usingtheMTTviabilityassayisdetermined(SeeMatTekEpiDermFTuseprotocol)foreachlotoftissue.ET-50’sgenerallyfallwithintherangeof6.5-9.5hours.ET-50’sincustomers’labmaydifferslightlyfromtheMatTekresults
Sterility: Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontamination
Screeningforpathogens: AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“Biosafetyinmicrobiologicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalservice
Notificationoflotfailure: IfatissuelotfailsourQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutchargewhenappropriate.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossible(Undernormalcircumstances,ET-50failureswillbenotifiedbyWednesday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)
