Dendriticcells(DC)playakeyroleintheimmunologicalreactionsthroughoutthebody.DCarehighlyspecializedantigen-presentingcells(APC)locatedintheskin,mucosa,andlymphoidtissues.DCplayakeyroleintheinductionphaseofcontactallergenicity,andcanbeusedinvitrotostudy contactsensitizationandotherimmunologicalreactionsofthebody.
AutotologousTCells(TC-100-CRY&TC-100-RT)areavailableuponrequest.
ProductDescription:
MatTek’sDCsaregeneratedfromCD34+Progenitorcellsharvestedfromumbilicalcordblood. Theproprietarymethodusedgeneratesalargenumberofcellsfromeachindividualdonor,allowingformultipleexperimentsinwhichthecelloriginandfunctionalityareconstant. MatTek’sDCsexpressCD1aandHLA-DRandcanbemaintainedincultureforatleast14dayswithoutanysignificantchangesinsurfaceMarkerexpressionorfunctionalitywhenculturedwithDC-MMaccordingtoMatTek’sDCCulturingProtocol.
MatTek’sDCswereanalyzedusingtransmissionelectronmicroscopywhichshowthepresenceofBirbeckgranules,akeyultrastructuralmarkersofDCsandLCs. UponstimulationwithLPSandPMA,areproducIBLeandsignificantinductionisobservedforIL-12,MIP-1α,MIP-3αandTNFαatthegeneandproteinexpressionlevels. FurThermore,MatTek’sDCsareinfectablewithHIVandstimulateheterologousTCells(TC)andautologousTCsfollowingexposuretoantigen.
Specifications:
Allcellsarenegativeformycoplasma,bacteria,yeast,andfungi.HIV-1,hepatitisBandhepatitisCarenotdetectedbyPCRforalldonorsandcelllots. Thisproductisforresearchuseonly. Notforuseinanimals,humansordiagnosticpurposes.
